I mea e mālama ai i nā huahana meaola maʻalahi no ka manawa lōʻihi, ʻo kahi ʻōmole cryogenic Dewar kahi ʻōnaehana e hāʻawi i kahi wahi wela haʻahaʻa paʻa e mālama i ke ola o nā huna nāwaliwali. ʻO Cryogenic Dewar kahi ʻano o ka moku kaomi ʻole, hoʻolālā ʻia a hana ʻia, hiki ke kū i nā mea cryogenic e pili ana i ka nitrogen wai. ʻAno ʻole ke kala o ka nitrogen wai, kala ʻole, ʻono ʻole, a hoʻonāukiuki ʻole hoʻi; no laila, ʻaʻohe ona ʻano laha a pono e lawelawe ʻia me ka mālama. I ke ana wela o - 196 ℃, manaʻo ʻia ka nitrogen wai he wai cryogenic, hiki ke hoʻohana ʻia e mālama i nā meaola i kaupalena ʻia.
ma muli o ke ola o ka nitrogen wai, hiki i ka cryopreservation ke hiki. Ma o ka mālama lōʻihi ʻana o nā hunaula, nā aʻa a me nā laʻana ʻē aʻe i nā ʻōmole cryogen Dewar, hiki ke hoʻomohala hou ʻia nā hana olakino a me ka noiʻi.
ʻo kēia mau mea he ʻelima mau pale e pale ai i ka dewar cryogenic a me nā mea i loko.
1. E hoʻohana i ka ʻōnaehana nānā pono i ka wela. I mea e pale aku ai i nā hopena biochemical e hiki ai ke hoʻohaʻahaʻa i ka cell, pono e mālama ʻia nā huahana biological maʻalike loa i nā mahana haʻahaʻa loa i nā dewar cryogenic. 2. Hiki i ka mahana mālama haʻahaʻa (eg - 196? C) ke mālama i ke ola i nā meaola ola. ʻO ke ala kūpono e hōʻoia ai i ka palekana o nā ʻike Dewar haʻahaʻa o ka mahana haʻahaʻa a mālama i ka mahana haʻahaʻa e hoʻokō i kahi ʻōnaehana nānā pono wai nitrogen.
3..E mālama i ka mahana haʻahaʻa Dewar i luna i nā manawa āpau. Pono e mālama pono i nā dewar Cryogenic i nā manawa āpau e hōʻoia i ka mālama pono ʻana. ʻO ka hoʻolei ʻana i ke dewar a i ʻole ke kau ʻana iā ia ma kona ʻaoʻao ke kumu e hoʻonui i ka wai hauota. ʻO ka hōʻino ʻana i ke dewar a i ʻole kekahi mea i mālama ʻia i loko o ia e kū pū kekahi.
4..No kūleʻa lawelawe. Hiki i ka lawelawe lima ʻana ke hōʻino loa i nā ʻōmole ʻo kūhōhō cryogen Dewar a me nā ʻike o loko. E hoʻokuʻu i ka ʻōmole Dewar, e hoʻohuli iā ia ma kona ʻaoʻao, a e ʻeha i ka hopena a me ka haʻalulu nui, i alakaʻi ʻia ai i ka nalo hapa a piha paha o ka ʻōpala. Hoʻemi ka ʻōnaehana hoʻowahāwahā i ka hoʻoili hoʻoili wela o ka wai cryogenic a mālama i ka dewar i ka mahana haʻahaʻa i nā manawa āpau. Hiki i ka mahana haʻahaʻa paʻa ke hālāwai i ka ikaika o ka noi haʻahaʻa haʻahaʻa.
5..E mālama a hoʻomaʻemaʻe i ka hāmeʻa. E hoʻokomo i ka hāmeʻa ma kahi maʻemaʻe a me kahi maloʻo. Paipai ka pulu, nā kemika, nā mea hoʻomaʻemaʻe ikaika a me nā mea ʻē aʻe i ka pala a pono e hemo koke. E hoʻomaʻemaʻe wale i ka hue Dewogen cryogen me ka wai a i ʻole ka detergent akahai a hoʻomaloʻo maloʻo loa e pale aku ai i ka pala o ka iwi metala. ʻO ka hōʻino ʻana i nā mea i hoʻohana ʻia e hana ke dewar hiki ke waiho i kahi mea i mālama ʻia i ka makaʻu.
E mālama pono i ka ea. ʻAʻole pono e uhi ʻia a hoʻopaʻa ʻia nā puka komo o kekahi Dewar cryogenic e pale i ka hoʻopilikia ʻana i ke kinoea. ʻAʻole kaomi ʻia nā dewars, no laila ʻaʻole lawa ka ea i hiki ke hopena i ke kaomi kōpena nui. ʻO kēia ke kumu o ka haki ʻana o ka ʻōmole Dewar a lilo i mea weliweli palekana no nā limahana a me nā meaola i mālama ʻia.
Ka manawa Post: Nov-09-2020